

It can, thus, further enhance the resolution of unknown metabolites, particularly for isomer metabolites and poor chromatographic separation. Moreover, MS is preferred for its excellent sensitivity, high specificity, and simplicity without elaborate sample preparatory procedures. However, compared to Liquid Chromatography-Mass Spectrometry (LC-MS), NMR is deemed less sensitive. This analytical method allows a wide range of metabolites to be identified simultaneously, offering an accurate and easily reproducible representation of the plant’s metabolome. Several analytical methods are applied in metabolomics, however, compared to other methods, proton nuclear magnetic resonance ( 1H-NMR) is widely used. Metabolomics provides insights into the fundamental nature of plant phenotype in relation to development, physiology, and environment. There is a growing interest in using metabolomics technology because it does not require a priori knowledge of the species’ chemical composition. To date, there has been no documentation of metabolomics profiles linked to geographical origin and the impact of cultivation on Amaranthus species. Since various compounds in Amaranthus leaves have been linked to the plant’s health-promoting effects, it is important to better understand compound profiles depending on the conditions of cultivation and environmental factors. are largely gathered from the wild, even though few selected cultivation incidences have been recorded. Gallic acid, which is another constituent isolated from Amaranth, has been reported to have many beneficial effects, including antioxidant, anti-inflammatory and antineoplastic properties. Ferulic acid, which has been isolated from Amaranth, possesses anti-inflammatory, antioxidant, antimicrobial activity, anticancer, and antidiabetic effects. spinosus, demonstrated antidiabetic, antifungal, and antitumor properties. For an example, α-spinosterol and squalene, isolated from A. Many studies have reported that Amaranth leaves are a rich source of diverse bioactive compounds such as gallic acid, ferulic acid, and quercetin which contribute to its health promoting attributes. Įdible plants, especially leafy vegetables, also contain phytochemicals, which have potential to impact human health positively. In South Africa, the leaves are favored to supplement starch-based diets.

In addition, the vegetables are packed with dietary fiber and minerals, such as magnesium, calcium, potassium, copper, phosphorus, zinc, iron, and manganese. Amaranth leaves are rich economic sources of carotenoids and proteins, including the essential amino acids methionine and lysine. Amaranth is not only rich in macro and micronutrients, but the leaves also contain phytochemicals that vary between species and cultivated plants, and might, therefore, affect the medicinal properties of the material.Īmaranthus has been rediscovered as a promising food crop mainly due to its resistance to biotic and abiotic factors and the high nutritional value of both seeds and leaves. On the contrary, L-tryptophan, kaempferol, phenylalanine and quercetin were detected only in wild A. hybridus, while chlorogenic acid was identified only in cultivated A. LC-MS analysis revealed the presence of rutin, 2-phenylethenamine and amaranthussaponin I in both wild A. hybridus are trehalose, trigonelline, lactulose, betaine, valine, alanine, fumarate, formate and kynurenine. Other compounds that were found in both wild and cultivated A. cruentus in high quantity whereas, proline and leucine were prominent in A. Moreover, the amino acid, proline was present in cultivated A. Through NMR analysis, it was found that maltose and sucrose increased in both cultivated A. hybridus were subjected to Liquid Chromatography-Mass Spectrometry (LC-MS) for further analysis. hybridus by multivariate statistical analysis of spectral data deduced by Nuclear Magnetic Resonance (NMR). This research investigated the effect of cultivation and environment on the chemical profile of both cultivated and wild A. They are mostly harvested from the wild, and cultivation of Amaranthus species is still rare, and therefore, attempts are being made to commercialize and market this important crop. Amaranthus crops are important for their use as food and nutritional sources, as well as for their medicinal properties.
